Electronic cigarettes, also known as electronic nicotine delivery systems (ENDS), are battery powered devices that are designed to deliver nicotine by heating a liquid solution (vape juice), creating an aerosol that is inhaled by users. Electronic cigarettes are advertised as a safer alternative to smoking traditional cigarettes (TCs) as well as an effective cessation tool. This is because the vape juice solution usually contains less chemicals than TCs. However, vape juice may contain carcinogenic chemicals such as formaldehyde, acrolein, and pulegone. Smoking has long been established as a major risk factor for developing esophageal cancer, but research on the impacts of vaping is limited. The objective of this study was to determine if human esophageal epithelium demonstrate carcinogenic properties after exposure to vaping. Esophageal keratinocytes exposed to vape-treated media had a decreased rate of proliferation and viability in comparison to untreated cells. This contradicts prior research on oral cancerous epithelial cells which found increased proliferation rates and viability after exposure to vaping. These results suggest that acute exposure to vaping does not cause esophageal keratinocytes to become carcinogenic. Future research should expand on this study to determine if the trends apparent after acute exposure to vaping are also apparent after long-term exposure to vaping.